Cancer Cell Line Medium SF is a chemically defined and animal component-free medium for of adherent as well as suspension cell lines. The medium ideally functions as a medium for recombinant protein and high-density exosome production as well as for spheroid cell culture. Cell lines tested • CeP1 • CHO-K1 • HaCaT • HEK293 • HEK293-S (Suspension adapted)• U251 MG • U-2 OS • VERO • HROCs (Human colon carcinoma cell lines) Quality controlpH = 7.2 +/- 0.02 at 20-25°C. Each lot has been tested for sterility and absence of mycoplasma and bacteria. DisclaimerKeep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product. Shelf life of 1 year from the date of manufacture.Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances). If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature. DisclaimerThis product is for in-vitro use only. Not intended for clinical or diagnostic applications
Cancer Cell Line Medium SF is a chemically defined and animal component-free medium for of adherent as well as suspension cell lines. The medium ideally functions as a medium for recombinant protein and high-density exosome production as well as for spheroid cell culture. This medium comes without L-glutamin. Cell lines tested • CeP1 • CHO-K1 • HaCaT • HEK293 • HEK293-S (Suspension adapted)• U251 MG • U-2 OS • VERO • HROCs (Human colon carcinoma cell lines) Quality controlpH = 7.2 +/- 0.02 at 20-25°C. Each lot has been tested for sterility and absence of mycoplasma and bacteria. DisclaimerKeep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product. Shelf life of 1 year from the date of manufacture.Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances). If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature. DisclaimerThis product is for in-vitro use only. Not intended for clinical or diagnostic applications
Serum-free and xeno-free growth medium for glioblastoma cell lines. Tested with: · NCH612· NCH421K· NCH690 Quality control· pH = 7.2 +/- 0.02 at 20-25°C. · Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance · Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product. · Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances). · If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.· Shelf life is 8 weeks from the date of manufacture. Disclaimer· This product is for in-vitro use only. Not intended for clinical or diagnostic applications.
HMSC-MG, ready-to-useAnimal-component freeContains Glutamine This medium is defined, serum- and xeno-free based on the formulation of Standard E6 medium. It contains transforming growth factor β (TGF-β) and basic fibroblast growth factor (bFGF), therefore its shelf life is limited. hMSC-MG represents a cell culture medium which is suited for the propagation of human mesenchymal stem cells of various origin. The cell culture plates and flasks must be coated using Vitronectin or iMatrix.
Serum- and xeno-free medium for in vitro culture of keratinocyte cell lines. Tested with · HaCaT Quality control· pH = 7.2 +/- 0.02 at 20-25°C. · Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance · Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product. · Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances). · If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.· Shelf life is 8 weeks from the date of manufacture. Disclaimer· This product is for in-vitro use only. Not intended for clinical or diagnostic applications.
Serum-free and xeno-free cell growth medium for culture of suspension cell lines, especially hybridoma, T-cell lines and B-cell lines. Tested with · Jurkat E6.1· NFS-60· WEHI-3B· WIL2· TK6· B-LCL lines Quality control· pH = 7.2 +/- 0.02 at 20-25°C. · Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance · Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product. · Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances). · If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.· Shelf life is 8 weeks from the date of manufacture. Disclaimer· This product is for in-vitro use only. Not intended for clinical or diagnostic applications.
SF-9 Cell Line Medium is a serum- and protein-free medium optimized for the growth of insect cells like Sf9 and Sf21 (Spodoptera frugiperda). SF-9 Cell Line Mediumhas been developed for the cultivation of insect cells and is suitable for production of recombinant proteins (Baculovirus expression vector system). Usage instructionsYou can follow this protocol to adapt your insect cell line to protein-free culture in Serum-free medium, for SF-9 cells. The optimal temperature range for most insect cells in 25°C to 30°C (27°C incubation +/- 0.5°C). The pH for cell culture with Sf9, Sf21 should be between pH 6.0 and pH 6.4, the osmolality should be 345-380 mOsm/kg. For oxygen supply, use vessels with filter lids or slightly unscrew the lids. For adaption from serum-containing to protein-free culture, direct or sequential adaption can be done. Cells should be taken from the middle exponential growth phase with >90% viability. Direct adaptation · Transfer cells from serum-containing culture directly into SF-9 Cell Line Medium (prewarmed to ambient temperature) with a density of 0.5 million cells/ml. · Subculture cells at > 2Million cells/ml, seed into fresh SF-9 Cell Line Medium with a density of 0.5 million cells/ml. · Repeat the subculture routine till the cells reach > 80% viability. Indirect adaptation · Start with subculturing the cells into a 1+1 mixture of the serum-containing medium and serum-free medium, for SF-9 cells, with a density of 0.5 million cells/ml. · At > 1 million cells/ml, dilute to 0.5 million cells/ml by adding the respective volume of serum-free medium, for SF-9 cells. · Repeat this process till serum levels below 0.1% is reached; the viability should be above 80%. Cell density should exceed 1 million cells/ml. Quality control· pH = 7.2 +/- 0.02 at 20-25°C. · Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance · Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product. · Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances). · If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.· Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture. Disclaimer· This product is for in-vitro use only. Not intended for clinical or diagnostic applications. Ingredients SF-9 Cell Line Medium contains amino acids, vitamins, salts, trace elements, lipids and growth promoting factors in a formulation optimized for insect cells. It is free of proteins and any components of human origin.
Serum-free cell growth medium for the cell line Vero. The low protein content (<2 mg/L) facilitates recombinant protein and/or virus production. Tested with · Vero· Vero-CDM Quality control· pH = 7.2 +/- 0.02 at 20-25°C. · Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance · Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product. · Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances). · If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.· Shelf life is 1 year from the date of manufacture. Disclaimer· This product is for in-vitro use only. Not intended for clinical or diagnostic applications.
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