Go to homepage
My account
Login
or sign up
Overview Your profile Addresses Payment methods Orders
Shopping cart €0.00*
Go to homepage
Products
Service
Resources
About CLS
Show all Products
Cell lines
Human cell lines
Breast cancer cell lines
B lymphoblastoid cell lines
Burkitt Lymphoma cell lines
Urinary bladder cancer cell lines
HaCaT cells and derivatives
Skin cancer cell lines
Brain cancer cell lines
Intestine cancer cell lines
Head-Neck cancer cell lines
Liver cancer cell lines
Leukemia cell lines
Lung cancer cell lines
Bone cancer cell lines
Lymphoma
Gastric cancer cell lines
Myeloma cell lines
Adrenal gland cancer cell lines
Neuroblastoma cell lines
Pancreas cancer cell lines
Prostate cancer cell lines
Reproductive system cancer cell lines
Kidney cancer cell lines
Soft tissue cancer cell lines
Animal cell lines
Monkey cell lines
Avian cell lines
Bovine cell lines
Canine cell lines
Hamster cell lines
Insect cell line
Mouse cell lines
Rat cell lines
Potoroo cell lines
Opossum cell line
Pig cell lines
Fluorescence tagged cell lines
HeLa Kyoto cells fluoreszence labeled
HeLa R19 Flpln TREXs
NRK cells fluorescence tagged
U-2 OS genome edited cell lines
Human primary cells
Mesenchymal Stem cells
Fibroblasts
HUVEC
Oral cells
Immortalized primary cells
Media & supplements
Dissociation solutions
Cryopreservation media
iPSC media
Buffer and solutions
Serum free cell culture media
Cell culture media
Basic media
Ready-to-use media
DNA & cell pellets
DNA
Cell pellets
Show all Service
Mycoplasma detection test
Cell line authentication
Cell banking
Show all Resources
FAQs
CoA
Discovering cell culture
HLA typed cancer cells
Show all categories iPSC media Back
  • Show iPSC media
    You are here:
  1. Products
  2. Media & supplements
  3. iPSC media
  • Products
    • Cell lines
    • Human primary cells
    • Media & supplements
      • Dissociation solutions
      • Cryopreservation media
      • iPSC media
      • Buffer and solutions
      • Serum free cell culture media
      • Cell culture media
    • DNA & cell pellets
  • Service
  • Resources
  • About CLS
Filter
–
iPSC Growth Medium Kit
iPSC Growth Medium Kit
This medium is defined, free of serum or Xeno additives and based on the formulation of the E6 medium but modified for increased performance of iPSCs. iPSC growth medium represents a cell culture medium that is suited for the propagation of human induced pluripotent stem cells of various origins. Upon supplementation, it should be used up within 2 weeks due to limited shelf life. It is recommended to prepare the medium for cell culture in aliquots unless the volume of 500 ml will be used within this time range. This medium is tested with iPS cells cultured on Vitronectin and iMatrix-coated 6-well-plates.

iPSC colonies

Figure 1: Examples of induced-pluripotent cells, iPSC-ASC (left) and iPSC-hDPSC (right), the latter derived by reprogramming Mesenchymal Stromal cells isolated from human Dental Pulp cells, cultivated in the CLS iPSC growth medium.

Pluripotency of cells cultured in iPSC growth medium
1. Flow cytometry data

Figure 2: Pluripotency of iPSC-hDPSC (clone 2) by flow cytometry: the cells were propagated in iPSC growth medium in 6-well-plates, detached using Accutase and stained. A) SOX-2, B) Oct3-4, C) Nanog.

2. Immunocytochemistry

Figure 3: Pluripotency of iPSC-hDPSC (clone 1) by immunocytochemical staining: iPSC-hDPSC derived by reprogramming Mesenchymal Stromal cells isolated from human Dental Pulp cells, cultivated in the iPSC growth medium in glass bottom dishes (ibidi). iPSCs expressed the pluripotency markers Oct3/4, SSEA4, TRA-60-1 and SOX2, staining was conducted using the PSC Immunocytochem Kit 4-Marker (Thermofisher).
Media preparation

The supplement comes in two parts, the basic medium and the supplement comprising very sensitive ingredients.
Due to the reduced shelf life of the media once supplemented, it is recommended to prepare smaller quantities such as 100 mL.
To prepare 100 mL of the iPSC growth medium, thaw the supplement at room temperature (18-25°C) or overnight at 4-8°C. Do not thaw at 37°C in a water bath, as this may be harmful to the ingredients. Aliquot the remaining 40 mL adequately and freeze down immediately.
Aseptically transfer 90 mL of the iPSC-MG basic medium into a sterile 100 mL bottle and add 10 mL of the supplement. Mix thoroughly. This volume should be used up within 1-2 weeks.
If other volumes of media are required, prepare accordingly.
For sterile filtration purposes, use 0.2 µm low protein binding polyethersulfone (PES) filter units (e.g. Corning Cat # 431229).

Maintenance

Keep the basic medium and the supplemented medium refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimizes the quality of the product.
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Store the supplement at -20°C. Do not leave it for a prolonged time at ambient temperature.
After supplementation, this medium has a shelf life of about two weeks.

€195.00*


Select your language

I want to use the website with the following language:
Service hotline

Support and counselling via: +49 6221 405780 Mon-Fri, 9 am - 4 pm

Or via our contact form.
Infos
  • Support
  • Ordering information
  • Payment and shipping costs
  • Distributors
CLS
  • Quality management
  • GTC
  • Legal notice
  • Contact
  • Newsletter
  • Book an appointment
Policies
  • Privacy policy
  • Technology transfer
  • Product use policy
  • Cookies & Tracking
Our communities
  • LinkedIn
Quality management
SSL Custom image 1
Newsletter

Just subscribe to our newsletter and you will always be among the first to be informed about new products and offers.

Privacy *

* All prices excl. VAT plus shipping costs and possible delivery charges, if not stated otherwise.

© 2023 CLS Cell Lines Service GmbH - All Rights Reserved.
Foundation Modal

This Modal is powered by moori Foundation

This website uses cookies to ensure the best experience possible. More information
Request sent successfully
Request wasn't send - a error occured. Please try again.

Request an offer

Request an offer for the choosen products

Product(s) in this request:
Please enter a valid email address.
Fields marked with * are mandatory.