RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3
RPMI 1640, also known as RPMI-1640 or RPMI medium, is a culture medium widely used in cell biology research. It was originally developed by Roy Merskey and Arthur Pappenheimer at Roswell Park Memorial Institute (RPMI) in the early 1960s. RPMI 1640 contains an optimal balance of essential minerals, amino acids, vitamins, and glucose for mammalian cells to thrive. It is a salt solution with a buffer made mostly of sodium chloride and dipotassium phosphate. To keep it stable, magnesium sulfate and calcium chloride were added. Additionally, it includes L-glutamine and 10% heat-inactivated fetal bovine serum (FBS). RPMI 1640 has been widely adopted as a versatile standard medium for cell culture.
It is suitable for a range of applications, such as culturing primary cells, stem cells, and immortalized cell lines. RPMI 1640 is also used in various immunological assays, such as ELISAs and co-cultures. The RPMI-1640 medium has been validated extensively over the years to ensure its reliability and consistency. It is recommended by experts in the field of cell biology for use in experiments requiring robust growth and proliferation of mammalian cell types. Its composition makes RPMI 1640 an ideal choice for many complex research applications.
1) Merskey, R., & Pappenheimer, A. M. (1963). A New Medium For The Cultivation Of Leukemia Cells And Other Tissue-Culture Cells. Proceedings of the Society for Experimental Biology and Medicine, 112(2), 845–849.
2) RPMI-1640: a protein-free medium optimized for growth characteristics of human hematopoietic cells. (1984). In Vitro Cellular & Developmental Biology, 20(4), 467–475.