SCC-25 Cells
General information
Description | SCC-25 is an epithelial-like cell line derived from the tongue of a patient with squamous cell carcinoma. |
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Organism | Human |
Tissue | Tongue |
Disease | Squamous cell carcinoma |
Synonyms | SCC 25, SCC25, hSCC-25 |
Characteristics
Age | 70 years |
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Gender | Male |
Ethnicity | European |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SCC-25 (Cytion catalog number 300607) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | epidermal keratins, low levels of involucrin |
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Tumorigenic | Yes, in nude mice. |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 20% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10
D13S317: 13
D16S539: 11,12
D5S818: 12
D7S820: 12
TH01: 8
TPOX: 8,12
vWA: 17,19
D3S1358: 17
D21S11: 30
D18S51: 16
Penta E: 14,15
Penta D: 13
D8S1179: 13
FGA: 20,24
D6S1043: 13,18
D2S1338: 17,19
D12S391: 17
D19S433: 13,14
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