SW-982 genomic DNA - 5 microgram

€95.00*

Content: 1 cryovial
Product variants
Product number: 300404GD5
Product nr Options Price
300404 cryopreserved culture €340.00
300404CP cell pellet - 1 million cells €75.00
300404CP5 cell pellet - 5 million cells €300.00
300404GD5 genomic DNA - 5 microgram €95.00
330404 growing culture €600.00
Product information "SW-982 genomic DNA - 5 microgram"

Manufacturing method

The genomic DNA (gDNA) is isolated by cell lysis followed by the addition of Proteinase K and RNAse A and purification on columns. The conditions have been chosen to allow for PCR or other enzymatic reactions in the downstream process. The fragment length of the purified DNA is up to 50kb.

Applications

  • PCR / RT-PCR / qPCR
  • Next-generation sequencing (NGS)
  • Single nucleotide polymorphism (SNP) analysis
  • Genomic analysis
  • Gene expression studies
  • Southern Blot

Concentration

50-100 ng/µl in TE Buffer (10 mM Tris-CL, 0.5 mM EDTA, pH 8.0). If you require a specific concentration, please contact us.

Quality control

  • The quality and purity of the DNA are tested with a spectrophotometer. Absorption at A260/280 is between 1.8 and 2.0. Contamination with RNA, proteoglycans, and polysaccharides is excluded.
  • We can quantify the double-stranded DNA (dsDNA) content free of charge, please contact us for more information.
  • Please contact us for further information if you require DNA from HLA-typed cell lines.
  • Store at 4°C for frequent use or at -20°C for occasional use.
  • For prolonged storage (> 6 months) we recommend -80°C.
  • Avoid more than three freeze/thaw cycles.

Storage

  • Store at 4°C for frequent use or at -20°C for occasional use.
  • For prolonged storage (> 6 months) we recommend -80°C.
  • Avoid more than three freeze/thaw cycles.

Note

Please centrifuge before opening the vial.

References "SW-982 genomic DNA - 5 microgram"
Contamination-free cells
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Bulk order
Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Authenticated cells
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA alleles
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

Required products

DMEM High Glucose
Supplemented with 4.5 g/l glucose, L-glutamine and 10% FBS. The shelf life of this ready-to-use medium is 8 weeks from the date of manufacture. 

Variants from €18.00*
€46.00*
Freeze Medium CM-1
- Ready-to-use and easy-to-use
- Suitable for long-term cryopreservation
- Tested with more than 400 cell lines
- Stable for more than 6 monthsCM-1 represents a cryopreservation medium developed by CLS enabling the long-time cryopreservation of Human and Animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, therefore consistant results with respect to cell viability following the thawing procedure are at hand. 

Variants from €59.00*
€39.00*
Accutase
Accutase Cell Detachment solution is a ready-to-use cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware, and polymers. Accutase performs exceptionally well in detaching cells for the analysis of cell surface markers, virus growth assays, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.Accutase does not contain mammalian or bacterial derived products.  

€43.95*
FBS
Our Fetal Bovine Serum (FBS) is triple filtered through 0.1 µm filters. Each lot is tested for sterility and for the ability to support the growth of several different cell lines. Fetal bovine serum is manufactured from fetal bovine blood collected at government registered facilities in South America. 
 




Cell lines cultured using FBS















2106T


VERO


FRTL-5




 
Quality control

Each lot has been tested for sterility and absence of mycoplasma and bacteria. 

 
Maintenance 

Keep frozen at -40°C to -10°C. 
The shelf life is 60 months.
Do not heat to more than 37°C and do not use uncontrollable sources of heat (e.g., microwave appliances). 

 
Disclaimer

This product is for in-vitro use only. Not intended for clinical or diagnostic applications.

 
 
 

 

Variants from €25.20*
€219.00*
PBS
Phosphate buffered solutionSterile, applicable in cell culture 

€7.00*