Our cell banking service is the ideal solution for your valuable human or animal cells, either derived from the tissue or blood of patients or healthy individuals, or already established as cell lines.  Advantages of our service- Standardized price for the storage of up to 10 cryovials (minimum amount) per year- Gentle freezing process in liquid nitrogen - Confidential storage- Shipment of stored cells at any point (surcharge)- Expansion of your cells on demand (surcharge)- Providing you with fresh vital cells in culture on demand (surcharge)- Identity (STR analysis) and mycoplasma testing (surcharge) Requirements- Cells have to be pre-tested for absence of mycoplasma (Service can be performed at CLS)   

The authentication of human cell lines which are being used in many scientific research projects is an important issue. To exclude any cross contamination of your valuable cell line, it is recommended to authenticate by STR analysis to detect misidentified cell lines. Our service includes- STR analysis- Comparison with online data bases- Publication-ready analysis report If you are sendinggDNA - please provide us with ≥50 μl of 50ng/μl gDNA in Tris or EDTA (10 mM Tris, 0.1 mM EDTA. DNA samples should be sent in a padded envelope at room temperature. Cell pellet - please provide us with 1-5 Mio. cells as a cell pellet. Please wash twice with PBS and resuspend in 0.5 ml of 70-90% ethanol. Please send in a padded envelope at room temperature. Results - You will get the STR profile within 2 weeks per email. The results include the comparison of the data with online databases and our own databases. The cell line will be classified as authenticated or misidentified.  Please note that non-European customers may have to pay for customs clearance and veterinary inspection. 

The authentication of murine cell lines which are being used in many scientific research projects is an important issue. To exclude any cross contamination of your valuable cell line, it is recommended to authenticate the cells to find misidentified cell lines. Our service includes- STR analysis- Comparison with online data bases- Publication-ready analysis report If you are sendinggDNA - please provide us with ≥50 μl of 50ng/μl gDNA in Tris or EDTA (10 mM Tris, 0.1 mM EDTA. DNA samples should be sent in a padded envelope at room temperature. Cell pellet - please provide us with 1-5 Mio. cells as a cell pellet. Please wash twice with PBS and resuspend in 0.5 ml of 70-90% ethanol. Please send in a padded envelope at room temperature. Results - You will get the results within 2 weeks per email. The results include the comparison of the data with online databases and our own databases. The cell line will be classified as authenticated or misidentified.   Please note that non-European customers may have to pay for customs clearance and veterinary inspection.

Mycoplasmas are small, prokaryotic organisms which are a frequent contamination of cell cultures. Mycoplasma are not visible by microscopic analysis. However, the contamination may affect the vitality of cell lines which slows down the proliferation rate and morphology. Approximately 15 -35% of cell cultures are contaminated. Therefore it is essential that all cell stocks are routinely checked for mycoplasmas to secure a mycoplasma-free environment.We conduct highly sensitive, specific cell-based as well as PCR-based tests for the most common species of mycoplasma, such as M. Hyorhinis, M. Arginini, M.Orale, M.Fermentans and A. laidlawii which represent 98% of tissue culture infections. The Mycoplasma Long Assay serves to detect mycoplasma during antibiotics-free cell cultivation at 37°C for 14 days via mycoplasma-specific qPCR and a sensitive cell-based test.  

Mycoplasmas are small, prokaryotic organisms which are a frequent contamination of cell cultures. Mycoplasma are not visible by microscopic analysis. However, the contamination may affect the vitality of cell lines which slows down the proliferation rate and morphology. Approximately 15 -35% of cell cultures are contaminated. Therefore it is essential that all cell stocks are routinely checked for mycoplasmas to secure a mycoplasma-free environment.We conduct highly sensitive, specific cell-based as well as PCR-based tests for the most common species of mycoplasma, such as M. Hyorhinis, M. Arginini, M.Orale, M.Fermentans and A. laidlawii which represent 98% of tissue culture infections. 

The purity of human and animal cell lines, which are being used all over the world in basic research, is prerequisite for good results. A simple and reliable method to exclude cross contaminations with other mammalian cells is Real-Time PCR. The following species can be determined as complete panel, parts of one panel or as single test:- Homo sapiens (human)- Mus musculus (mouse)- Rattus norvegicus (rat)- Chlorocebus aethiops (african green monkey)- Canis lupus familiaris (dog)- Bos Taurus (cattle)- Sus scrofa (pig)- Oryctolagus cuniculus (rabbit)- Gallus gallus (chicken)- Cricetulus griseus (Chinese hamster)- Mesocricetus auratus (Syrian hamster)  

The purity of human and animal cell lines, which are being used all over the world in basic research, is prerequisite for good results. A simple and reliable method to exclude cross contaminations with other mammalian cells is Real-Time PCR. The following species can be determined as complete panel, parts of one panel or as single test:- Homo sapiens (human)- Mus musculus (mouse)- Rattus norvegicus (rat)- Chlorocebus aethiops (african green monkey)- Canis lupus familiaris (dog)- Bos Taurus (cattle)- Sus scrofa (pig)- Oryctolagus cuniculus (rabbit)- Gallus gallus (chicken)- Cricetulus griseus (Chinese hamster)- Mesocricetus auratus (Syrian hamster)