Sf9 genomic DNA - 5 microgram

€95.00*

Content: 1 cryovial
Product variants
Product number: 604328GD5
Product nr Options Price
604328 cryopreserved culture €340.00
604328CP cell pellet - 1 million cells €75.00
604328CP5 cell pellet - 5 million cells €300.00
604328GD5 genomic DNA - 5 microgram €95.00
664328 growing culture €600.00
Product information "Sf9 genomic DNA - 5 microgram"

Manufacturing method

The genomic DNA (gDNA) is isolated by cell lysis followed by the addition of Proteinase K and RNAse A and purification on columns. The conditions have been chosen to allow for PCR or other enzymatic reactions in the downstream process. The fragment length of the purified DNA is up to 50kb.

Applications

  • PCR / RT-PCR / qPCR
  • Next-generation sequencing (NGS)
  • Single nucleotide polymorphism (SNP) analysis
  • Genomic analysis
  • Gene expression studies
  • Southern Blot

Concentration

50-100 ng/µl in TE Buffer (10 mM Tris-CL, 0.5 mM EDTA, pH 8.0). If you require a specific concentration, please contact us.

Quality control

  • The quality and purity of the DNA are tested with a spectrophotometer. Absorption at A260/280 is between 1.8 and 2.0. Contamination with RNA, proteoglycans, and polysaccharides is excluded.
  • We can quantify the double-stranded DNA (dsDNA) content free of charge, please contact us for more information.
  • Please contact us for further information if you require DNA from HLA-typed cell lines.
  • Store at 4°C for frequent use or at -20°C for occasional use.
  • For prolonged storage (> 6 months) we recommend -80°C.
  • Avoid more than three freeze/thaw cycles.

Storage

  • Store at 4°C for frequent use or at -20°C for occasional use.
  • For prolonged storage (> 6 months) we recommend -80°C.
  • Avoid more than three freeze/thaw cycles.

Note

Please centrifuge before opening the vial.

Contamination-free cells
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Bulk order
Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Authenticated cells
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA alleles
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

Required products

SF-9 Cell Line Medium
SF-9 Cell Line Medium is a serum
- and protein-free medium optimized for the growth of insect cells like Sf9 and Sf21 (Spodoptera frugiperda). SF-9 Cell Line Mediumhas been developed for the cultivation of insect cells and is suitable for production of recombinant proteins (Baculovirus expression vector system). Usage instructionsYou can follow this protocol to adapt your insect cell line to protein-free culture in Serum-free medium, for SF-9 cells. The optimal temperature range for most insect cells in 25°C to 30°C (27°C incubation +/
- 0.5°C). The pH for cell culture with Sf9, Sf21 should be between pH 6.0 and pH 6.4, the osmolality should be 345-380 mOsm/kg. For oxygen supply, use vessels with filter lids or slightly unscrew the lids. For adaption from serum-containing to protein-free culture, direct or sequential adaption can be done. Cells should be taken from the middle exponential growth phase with >90% viability.  Direct adaptation ·Transfer cells from serum-containing culture directly into SF-9 Cell Line Medium (prewarmed to ambient temperature) with a density of 0.5 million cells/ml. ·Subculture cells at > 2Million cells/ml, seed into fresh SF-9 Cell Line Medium with a density of 0.5 million cells/ml. ·Repeat the subculture routine till the cells reach > 80% viability.  Indirect adaptation ·Start with subculturing the cells into a 1+1 mixture of the serum-containing medium and serum-free medium, for SF-9 cells, with a density of 0.5 million cells/ml. ·At > 1 million cells/ml, dilute to 0.5 million cells/ml by adding the respective volume of serum-free medium, for SF-9 cells. ·Repeat this process till serum levels below 0.1% is reached; the viability should be above 80%. Cell density should exceed 1 million cells/ml.  Quality control·pH = 7.2 +/
- 0.02 at 20-25°C. ·Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance ·Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product. ·Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances). ·If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.·Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture. Disclaimer·This product is for in-vitro use only. Not intended for clinical or diagnostic applications. Ingredients SF-9 Cell Line Medium contains amino acids, vitamins, salts, trace elements, lipids and growth promoting factors in a formulation optimized for insect cells. It is free of proteins and any components of human origin.  

€50.00*
Freeze medium CM-ACF, serum free
- Ready-to-use and easy-to-use
- Free of serum and animal-derived components
- Suitable for long-term cryopreservation
- Tested with more than 400 cell lines
- Stable for more than 6 monthsCM-ACF is a cryopreservation medium enabling the long-time cryopreservation of human and animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, and consistent results with respect to cell viability following the thawing procedure are at hand.

€59.00*
Freeze Medium CM-1
- Ready-to-use and easy-to-use
- Suitable for long-term cryopreservation
- Tested with more than 400 cell lines
- Stable for more than 6 monthsCM-1 represents a cryopreservation medium developed by CLS enabling the long-time cryopreservation of Human and Animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, therefore consistant results with respect to cell viability following the thawing procedure are at hand. 

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Accutase
Accutase Cell Detachment solution is a ready-to-use cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware, and polymers. Accutase performs exceptionally well in detaching cells for the analysis of cell surface markers, virus growth assays, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.Accutase does not contain mammalian or bacterial derived products.  

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PBS
Phosphate buffered solutionSterile, applicable in cell culture 

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