|Description||The WI-38 cell line was the first human diploid cell line to be used to make vaccines for humans. It may have had a more significant impact on science and medicine than the well-known HeLa cell line. The cells were derived from normal embryonic lung tissue of a 3-month-old fetus, obtained from an elective abortion in Sweden in 1963. The WI-38 cells have been and still are used to produce a variety of vaccines for various virus-based diseases such as poliomyelitis, measles, mumps, rubella, varicella, herpes zoster, adenovirus, rabies, and Hepatitis A. Sanofi-Pasteur's Imovax rabies vaccine and Merck's chicken pox and shingles vaccines are grown in MRC-5 cells. Merck's rubella vaccine, Hepatitis A vaccines from both Merck and GlaxoSmithKline, the polio part of Sanofi Pasteur's Quadracel vaccine, and the U.S. military's adenovirus vaccine are all grown in WI-38 cells. WI-38 cells have a finite lifespan of roughly 50 population doublings, with a doubling time of 24 hours. They are susceptible to various viruses, including vesicular stomatitis, Glasgow (Indiana), herpes simplex virus, pseudorabies virus, and human poliovirus 1. The initials "WI" represent the Wistar Institute of Anatomy and Biology in Philadelphia, Pennsylvania, where the early work on the cell line was done.|
|Synonyms||Wi-38, WI38, Wistar Institute-38, AG06814E, AG06814G, AG06814H, AG06814-J, AG06814J, AG06814-M, AG06814-N|
|Age||3 months gestation|
Identifiers / Biosafety / Citation
|Citation||WI 38 (CLS catalog number 300428)|
Expression / Mutation / Susceptibility
|Medium supplements||10% FBS, 2.0 mM L-glutamin, 1.0 g/L L-glucose, 2.2 g/L NaHCO3|
|Freeze medium||CM-1 (CLS catalog number 800100) or CM-ACF (CLS catalog number 806100)|
|Handling of cryopreserved cultures||The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.|
|Handling of proliferating cultures||One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.|
Quality control / Genetic profile / HLA
|Sterility||Mycoplasma contamination was excluded through PCR-based and luminescence-based mycoplasma assays. Bacterial or fungal contaminations are detected through daily visual cell monitoring.|
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.