HuH7 cell pellet - 1 million cells

75,00 €*

Inhalt: 1 Kryovial
Produktvarianten
Produktnummer: 300156CP
Produkt-Nr Optionen Preis
300156 cryopreserved culture 540,00 €
300156CP cell pellet - 1 million cells 75,00 €
300156CP5 cell pellet - 5 million cells 300,00 €
300156GD5 genomic DNA - 5 microgram 95,00 €
330156 growing culture 800,00 €
Produktinformationen "HuH7 cell pellet - 1 million cells"

Manufacturing method

The cells have been cultured to about 80-90% confluence and harvested according to standard cell culture procedures. Detached cells were rinsed twice in ice-cold PBS, collected by centrifugation, and snap-frozen immediately in liquid nitrogen.

Applications

  • Isolation of gDNA
  • Isolation of RNA
  • Production of lysates
  • Fixation of the pellet and histochemistry

Concentration

2.0 million cells per pellet

Quality control

The cell preparation was free of mycoplasma contamination as determined through PCR. Cell viability was >90% ahead of preparing the aliquots.

Storage

Keep the cell pellet at -196°C for long-term storage. Do not store the pellets at -80°C for a longer period. Always keep the cell pellet on ice during preparations.

Note

Please centrifuge before opening the vial.

Referenzen "HuH7 cell pellet - 1 million cells"
Contamination-free cells
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Bulk order
Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Authenticated cells
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA alleles
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

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Freeze Medium CM-1
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FBS
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PBS
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