Glioblastoma is a highly malignant central nervous system tumor, representing over 60% of all brain tumors in adults. GBM is a severe medical condition that has been associated with poor prognosis. Scientists and researchers continuously study gliomas' biology to find effective treatments for patients. Glioblastoma Cell Growth Medium is a serum-free and xeno-free medium designed to support the growth of glioblastoma cell lines, including fibroblast cells.
Discover our Glioblastoma Cell Growth Medium
Glioblastoma Cell Growth Medium is a high-quality and ready-to-use medium that is specially formulated to support the growth and maintenance of glioblastoma cell lines. The medium is serum-free and xeno-free, which ensures the absence of animal-derived components. This medium is the successor to GBM-MG and has been adapted to support the growth of fibroblast cells, particularly human Gingival Fibroblasts.
Cells tested with our Glioblastoma Cell Growth Medium

NCH612
NCH421K
NCH690

Composition of the medium
Glioblastoma Cell Growth Medium contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, and trace minerals that support the proliferation of fibroblast cells. To promote adherence to the cell culture bottom, the supplement contains 2% FBS.
Quality Control
Each lot of Glioblastoma Cell Growth Medium undergoes a rigorous quality control process to ensure it meets the highest standards. The pH of the medium is maintained at 7.2 +/
- 0.02 at 20-25°C. Each lot is tested for sterility and the absence of mycoplasma and bacteria. This ensures that the medium is free from contamination and will provide consistent results.
Storage and Maintenance

Glioblastoma Cell Growth Medium should be stored refrigerated at +2°C to +8°C in the dark.
Freezing or warming up to +37°C can compromise the quality of the product.
To use the medium, remove the required amount from the bottle and warm it to room temperature. The shelf life of the product is eight weeks from the date of manufacture.

Disclaimer
Glioblastoma Cell Growth Medium is for in-vitro use only and is not intended for clinical or diagnostic applications. It is designed to be used by trained professionals and researchers in a laboratory setting.

SF-9 Cell Growth Medium is a serum
- and protein-free medium optimized for the growth of insect cells like Sf9 and Sf21 (Spodoptera frugiperda). SF-9 Cell
Growth

Medium has been developed for the cultivation of insect cells and is suitable for the production of recombinant proteins (Baculovirus expression vector system).
Usage instructions
You can follow this protocol to adapt your insect cell line to protein-free culture in a serum-free medium, for SF-9 cells. The optimal temperature range for most insect cells is 25°C to 30°C (27°C incubation +/
- 0.5°C). The pH for cell culture with Sf9, and Sf21 should be between pH 6.0 and pH 6.4, and the osmolality should be 345-380 mOsm/kg. For oxygen supply, use vessels with filter lids or slightly unscrew the lids. For adaption from serum-containing to protein-free culture, direct or sequential adaption can be done. Cells should be taken from the middle exponential growth phase with >90% viability.
Direct adaptation

Transfer cells from serum-containing culture directly into SF-9 Cell
Growth

Medium (prewarmed to ambient temperature) with a density of 0.5 million cells/ml.
Subculture cells at > 2 million cells/ml, seed into fresh SF-9 Cell
Growth

Medium with a density of 0.5 million cells/ml.
Repeat the subculture routine till the cells reach > 80% viability.

Indirect adaptation

Start with subculturing the cells into a 1+1 mixture of the serum-containing medium and serum-free medium, for SF-9 cells, with a density of 0.5 million cells/ml.
At > 1 million cells/ml, dilute to 0.5 million cells/ml by adding the respective volume of serum-free medium, for SF-9 cells.
Repeat this process till serum levels below 0.1% is reached; the viability should be above 80%. Cell density should exceed 1 million cells/ml.

Quality control

pH = 7.2 +/
- 0.02 at 20-25°C
Each lot has been tested for sterility and absence of mycoplasma and bacteria

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimizes the quality of the product.
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

Ingredients
SF-9 Cell
Growth

Medium contains amino acids, vitamins, salts, trace elements, lipids, and growth-promoting factors in a formulation optimized for insect cells. It is free of proteins and any components of human origin.
Disclaimer
This product is for in-vitro use only. Not intended for clinical or diagnostic applications.